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Products_type
Secondary Antibody
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Products_short_name
[IgG (H&L), F(ab)'2 Fragment]
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Products_name_syn
[Donkey anti-sheep IgG (H&L), F(ab)'2 Fragment DyLight 800-Conjugate]
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Reactivity
Sheep. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin Sheep serum proteins IgG from Human, Mouse, or Rabbit
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Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on Sheep IgG, light chains on all Sheep immunoglobulins
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Purity
> 90% based on SDS-PAGE, Small amounts of intact IgG may be present. Affinity purified using solid phase Sheep IgG.
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Form
Lyophilized
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Concentration
1.0 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store freeze-dried powder at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Latin name
Equus africanus asinus, Mus musculus, Oryctolagus cuniculus
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Short name
anti- IgG (H L), F(ab)'2 DyLight 800-Conjugate min x- w/ , Mouse, Rabbit IgG
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Technique
Rabbit, anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, mouses
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Host
Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
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Isotype
IgG, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. Heavy & Light chains, IgG
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Label
DyLight
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Species
Mouse, Donkeys, Humans, Mouses, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
Donkey Antibody tosheep Immunoglobulin G (heavy + light chain), F(Antibody)'2 Fragment DyLight 800-Conjugate min x- w/H. sapiens, Mouse, production species: rabbit Immunoglobulin G
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Alternative technique
rabbit-anti, murine, antibodies, immunoglobulins
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