Donkey Anti Human IgM Fluor (FITC)

• Catalog number
  MBS460704
• Price
  Please ask
• Size
  0.5 mg

• Products_type
  Secondary Antibody
• Products_short_name
  [IgM]
• Products_name_syn
  [Fluor (FITC) Conjugated Donkey Anti-Human IgM, F5u Fragment Specific]
• Other_names
  [IgM, partial]
• Products_gene_name
  [IgM]
• Reactivity
  Human
• Specificity
  The affinity purified antibody is labeled by Fluorescein Isothiocyanate (FITC). This antibody was raised in donkey by immunizing purified human IgM (F5u fragment specific). These antiserum were then immunoaffinity purified from the antigen specific column with minimum cross-reaction to bovine, mouse, and rabbit serum proteins. No antibody was detected against nonimmunoglobulin serum proteins.
• Form
  Sterile Liquid
• Storage_stability
  Keep the product at 2-8 degree C with sterile condition in the dark. For long term storage, add an equal amount of glycerol, mix and stored at -20 degree C. Alternatively, the product may be aliquoted and stored at -70 degree C or below without adding glycerol. Avoid freezing and thawing frequently.
• Tested_application
  Flow Cytometry (FC/FACS), Immunoassay
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
• Latin name
  Equus africanus asinus
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
• Conjugation
  Anti-FITC Antibody

• Gene target
  Fluor  
• Short name
  Anti IgM Fluor (FITC)
• Technique
  anti human, anti, FITC, IgM, antibody to, anti human antibodies, Fluorescein, IgMs
• Host
  Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
• Isotype
  IgM, IgM
• Label
  FITC
• Species
  Human, Donkeys, Humans
• Alternative name
  Donkey antibody to H. sapiens Immunoglobulin M Fluor (fluorecein)
• Alternative technique
  antibodies, fluorescine, immunoglobulin-m

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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