Cy3 Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]
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Catalog numberMBS417868
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Price:
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Size0.25 mg
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Products_typeSecondary Antibody
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Products_short_name[IgG [H&L]]
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Products_gene_name[IgG]
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ReactivityHuman
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Concentration1.0 mg/ml (by UV absorbance at 280 nm)
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Storage_stabilityFor extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
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Tested_applicationImmunomicroscopy, Flow Cytometry (FC/FACS), Other antibody based fluorescent assays requiring lot-to-lot consistency.
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TestA high affinity purification column was use to purify Conjugated anti- IgG [H L] [ ] by MyBioSource by chromatographic size exclusion. MyBioSource supplies antibodies that are for research of human proteins.
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Description1 of protein A or G purified by MyBioSource will give more specificity than crude serum. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesCy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Conjugated anti- IgG [H L] [ ] can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This MyBioSource antibody is well suited for fluorescent microscopy. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugationcy3 conjugation kit
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Latin nameEquus africanus asinus
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short nameConjugated anti- IgG [H L] [ ]
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Techniqueanti-Human, affinity, purified, anti-, anti, IgG, antibody to, antibodies, antibodies against human proteins, antibody Conjugates, IgGs, purifications
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HostDonkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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IsotypeIgG, Heavy & Light chains, IgG
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LabelCy3
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SpeciesHuman, Donkeys, Humans
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Alternative namecyanine 3 coupled protein enriched Antibody toHuman Immunoglobulin G [heavy + light chain] [Donkey]
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Alternative techniquepure, antibodies, immunoglobulins
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data