COMP (rat):Angiopoietin-1 (human), (recombinant) (FLAG)
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Catalog number
MBS566086
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Price
Please ask
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Size
0.5 mg
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Products_type
Recombinant Protein
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Products_short_name
[COMP]
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Other_names
[angiopoietin-1 isoform 2; Angiopoietin-1; angiopoietin-1; ANG-1; angiopoietin 1]
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Products_gene_name
[COMP]
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Other_gene_names
[ANGPT1; ANGPT1; AGP1; AGPT; ANG1; KIAA0003; ANG-1]
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Specificity
Binds to human Tie-2
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Purity
>=90% (SDS-PAGE)
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Form
Liquid. 0.2um-filtered solution in PBS containing 0.01% CHAPS
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Concentration
~0.2-0.4mg/mL
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Storage_stability
For long term storage, store at -80 degree CWorking aliquots are stable for up to 3 months when stored at -80 degree CAvoid freeze/thaw cycles. After opening, prepare aliquots and store at -80 degree CShipping: Shipped on Dry Ice
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Flag
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
COMP
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Short name
COMP ( ):Angiopoietin-1 ( ), (recombinant) ( )
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
CHO cells
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Label
Flag
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Species
Human, Humans, Rats
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Alternative name
cartilage oli this GO meric matrix protein (rat):Angiopoietin-1 (H. sapiens), (Rec.) (FLAG)
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Alternative technique
rec
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Alternative to gene target
cartilage oli this GO meric matrix protein, EDM1 and EPD1 and MED and PSACH and THBS5, COMP and IDBG-38816 and ENSG00000105664 and 1311, heparan sulfate proteoglycan binding, Extracellular, Comp and IDBG-162847 and ENSMUSG00000031849 and 12845, BT.3034 and IDBG-641613 and ENSBTAG00000004630 and 281088
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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