Cholera Toxin, beta (FITC)
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Catalog number
MBS642358
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Price
Please ask
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Size
5x1 mL
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Products_type
Antibody
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Products_short_name
[Cholera Toxin, beta]
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Products_name_syn
[Anti -Cholera Toxin, beta (FITC)]
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Other_names
[cholera toxin]
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Clonality
Polyclonal
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Specificity
Recognizes Beta subunit of Cholera toxin. Immunoprecipitates toxin in gel techniques.
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Purity
Highly Purified Purified IgG fraction to a purity of > 95%.
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Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with FITC.
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Storage_stability
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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Tested_application
ELISA (EL/EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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