Chicken anti Sheep IgG (H+L), conjugated with Biotin

Products_type

Secondary Antibody
Products_short_name

[IgG (H+L)]
Products_gene_name

[IgG]
Form

Liquid; 0.5 mL, Phosphate Buffered Saline‚ 50% Glycerol with 0.01% Thimerosal
Concentration

1.0 mg/mL
Storage_stability

Store at -20 degree C.
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
Conjugation

Biotinylated
About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Gene target

H+L conjugated with
Short name

anti IgG (H+L), conjugated with
Technique

anti, IgG, antibody to, antibody Conjugates, IgGs
Host

Chicken
Isotype

IgG, IgG
Label

Biotin
Species

Sheep, Chickens, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
Alternative name

Chicken antibody to ovine Immunoglobulin G (heavy + light chain), coupled including biotinilated
Alternative technique

antibodies, immunoglobulins

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data