Chagas 1F8, Recombinant, Trypanosoma cruzi

Products_type

Recombinant Protein
Products_short_name

[Chagas 1F8, Trypanosoma cruzi]
Purity

Affinity Purified~90-95% (SDS-PAGE 15%). Purified by metal affinity chromatography.
Form

Supplied as a lyophilized powder in 20mM HEPES, pH 8.0, 300mM sodium chloride, 284mM imidazole, 2% sucrose, ProClin 300. Reconstitute with 250ul sterile ddH2O through the septum of the tube. Centrifuge briefly (1000-2000 rpm for 5 minutes).
Storage_stability

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -70 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Chagas 1F8 Trypanosoma cruzi
Gene symbol

IL36B
Short name

Chagas 1F8, Recombinant, Trypanosoma cruzi
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Alternative name

Chagas 1F8, Rec., Trypanosoma cruzi
Alternative technique

rec

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data