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Products_type
Antibody
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Products_short_name
[CD53]
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Products_name_syn
[CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25); TSPAN25]
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Other_names
[leukocyte surface antigen CD53 isoform 1; Leukocyte surface antigen CD53; leukocyte surface antigen CD53; CD53 molecule; Cell surface glycoprotein CD53; Tetraspanin-25; Tspan-25; CD_antigen: CD53]
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Products_gene_name
[CD53]
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Other_gene_names
[CD53; CD53; MOX44; TSPAN25; MOX44; TSPAN25; Tspan-25]
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Clonality
Monoclonal
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Clone
[63-5A3]
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Reactivity
Human. Others not tested
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Specificity
Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.
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Form
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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Storage_stability
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Tested_application
Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
CD53
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Short name
CD53 (TSPAN25) Mouse Monoclonal Antibody
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
Mouse, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG2b, kappa
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Species
Mouse, Mouses
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Alternative name
CD53 molecule (TSPAN25) Mouse monoclonal (antibody to-)
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Alternative technique
antibodies, murine
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Alternative to gene target
CD53 molecule, MOX44 and TSPAN25, CD53 and IDBG-101004 and ENSG00000143119 and 963, protein binding, Cell surfaces, Cd53 and IDBG-184902 and ENSMUSG00000040747 and 12508, CD53 and IDBG-635180 and ENSBTAG00000006466 and 505040
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