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Products_type
Antibody
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Products_short_name
[CD20]
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Products_name_syn
[APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; Bp35; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane spanning 4 domains subfamily A member 2; Membrane-spanning 4-domains subfamily A member 1 (MS4A1)]
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Other_names
[B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1; membrane-spanning 4-domains, subfamily A, member 1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD_antigen: CD20]
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Products_gene_name
[MS4A1]
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Other_gene_names
[MS4A1; MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; CD20]
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Clonality
Monoclonal
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Clone
[B9E9]
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Reactivity
Human.
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Specificity
Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
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Form
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
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Storage_stability
Store at 2 to 8 degree C. Antibody is stable for 24 months.
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Tested_application
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies, Immunoprecipitation (IP), Immunohistology (IH)
