CD165 (AD2) (FITC)
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Catalog numberMBS600996
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PricePlease ask
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Size120 Tests
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Products_typeAntibody
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Products_short_name[CD165]
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Products_name_syn[Anti -CD165 (AD2) (FITC)]
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Other_names[cytidine and deoxycytidylate deaminase zinc-binding region family protein]
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ClonalityMonoclonal
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Clone[7H142]
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ReactivityHuman
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SpecificityRecognizes the CD165 molocule of 37kD.
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PurityAffinity Purified Purified by Protein A affinity chromatography.
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FormSupplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 5% glycerol, 0.2% BSA, 0.04% sodium azide.
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Storage_stabilityMay be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_applicationFlow Cytometry (FC/FACS)
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Gene symbolAPOE
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Short nameCD165 (AD2) (FITC)
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TechniqueFITC, Fluorescein
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HostMouse
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IsotypeIgG1
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LabelFITC
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Alternative nameCD165 (AD2) (fluorecein)
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Alternative techniquefluorescine
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Gene info
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Identity
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Gene
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Long gene nameapolipoprotein E
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Synonyms gene
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Synonyms gene name
- Alzheimer disease 2 (APOE*E4-associated, late onset)
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Apolipoproteins
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data