CD137(4-1BB)-huIg-FITC[CD137]

Products_type

Protein
Products_gene_name

[CD137]
Reactivity

Human
Properties

This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Gene target

CD137 4-1BB huIg [CD137]
Gene symbol

TNFRSF9, TNFSF9, MIR1302-4, PIRC18, PIRC16, SNORD114-4, IGKV2OR2-4, MIR941-4
Short name

CD137(4-1BB)-huIg-FITC[CD137]
Technique

FITC, Fluorescein
Host

CHO cells
Label

FITC
Alternative name

CD137(4-1BB)-huIg-fluorecein[CD137]
Alternative technique

fluorescine

Identity

HGNC:5809
Gene

IGKV2OR2-4
Long gene name

immunoglobulin kappa variable 2/OR2-4 (pseudogene)
Synonyms gene name
- immunoglobulin kappa variable 2/OR2-4
- immunoglobulin kappa variable 2/OR2-4 pseudogene
Synonyms
- IGKV2/OR2-4
GenBank acession
- X51883
Locus

2q11.2
Discovery year

2000-04-18
Entrez gene record

28858
Pubmed identfication
- 2111145
Classification
- Immunoglobulin kappa (IGK) orphons

Name

Fluorescent Antibody Technique
Concept

Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data