Biotin conjugated mAb anti-hGH

Products_type

Antibody
Products_short_name

[hGH]
Products_name_syn

[Human Growth Hormone (hGH) (**Biotin Labeled); Biotin conjugated mouse monoclonal antibody to human growth hormone (hGH)]
Products_gene_name

[hGH]
Clonality

Monoclonal
Clone

[YC8]
Reactivity

Human. Others not tested
Specificity

This monoclonal antibody reacts with natural and recombinant hGH. The affinity constant measured by Friguet's (1985) method is 1.7 x 1010/M.
Purity

Protein G affinity purified
Form

0.01M PBS pH 7.2 containing 1% gelatin and 0.1 % preclin-300
Storage_stability

Store at -20 degree C. Avoid repeated freeze and thaw cycles.
Tested_application

ELISA
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

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