Bilirubin Oxidase (Biotin)

Products_type

Antibody
Products_short_name

[Bilirubin Oxidase]
Products_name_syn

[Anti -Bilirubin Oxidase (Biotin)]
Other_names

[bilirubin oxidase]
Clonality

Polyclonal
Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as puri ed and partially puri ed Bilirubin Oxidase [Myrothecium verrucaria].
Purity

Highly Purified Puri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Storage_stability

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Tested_application

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated