Anti-Phospho-Ser9 Synapsin I

Products_type

Antibody
Products_short_name

[Synapsin I (Ser9)]
Other_names

[synapsin-1; Synapsin-1; synapsin-1; Synapsin I]
Products_gene_name

[SYN1]
Other_gene_names

[SYN1; SYN1]
Clonality

Polyclonal
Reactivity

Rat
Specificity

Specific for ~78k synapsin I doublet protein phosphorylated at Ser9. The antibody also weakly labels the ~55k synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with lambda phosphatase.
Purity

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Form

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Storage_stability

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Tested_application

Western Blot (WB), Immunohistochemistry (IHC)
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data