Anti-Phospho Ser477 Che-1 (AATF)

Products_type

Antibody
Products_short_name

[Che-1 (AATF) (Ser477)]
Other_names

[protein AATF; Protein AATF; protein AATF; rb-binding protein Che-1; apoptosis-antagonizing transcription factor; apoptosis antagonizing transcription factor; Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1]
Products_gene_name

[AATF]
Other_gene_names

[AATF; AATF; DED; BFR2; CHE1; CHE-1; CHE1; DED]
Clonality

Polyclonal
Reactivity

Human
Specificity

Specific for the ~66k Che-1 protein phosphorylated at Ser477. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide.
Purity

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Form

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Storage_stability

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Tested_application

Western Blot (WB)
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data