Anti-Mouse CD90 (Thy 1.2), FITC (Clone 5a-8) (mouse IgG2b)

  • Catalog number
    MBS520246
  • Price
    Please ask
  • Size
    0.1 mg
  • Products_type
    Antibody
  • Products_short_name
    [CD90 (Thy 1.2)]
  • Products_name_syn
    [CD90 (Thy 1.2), FITC (Clone 5a-8) (mouse IgG2b); FITC Anti-Mouse CD90 (Thy 1.2) Monoclonal Antibody]
  • Products_gene_name
    [CD90 (Thy 1.2)]
  • Clonality
    Monoclonal
  • Clone
    [5a-8]
  • Reactivity
    Mouse
  • Specificity
    CD90 (Thy 1.2) (Mouse CD90 (Thy 1.2))
  • Form
    FITC (FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Purified from ascitic fluid via Protein G Chromatography).)
  • Concentration
    Antibody Concentration: 0.1 mg/ml
  • Storage_stability
    Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles. Avoid prolonged exposure to light.
  • Tested_application
    Immunohistochemistry (IHC)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    CD90   Thy   5a-8   IgG2b  
  • Gene symbol
    THY1
  • Short name
    Anti-Mouse CD90 (Thy 1 2), FITC (Clone 5a-8) (mouse IgG2b)
  • Technique
    anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
  • Host
    Mouse
  • Isotype
    Mouse IgG2b, k, IgG2b
  • Label
    FITC
  • Species
    Mouse, Mouses
  • Alternative name
    Antibody toMouse CD90 (Thy 1.2), fluorecein (clonality 5a-8) (mouse IgG2b)
  • Alternative technique
    murine, antibodies, fluorescine
  • Clone name
    5a-8
Gene info
  • Identity
  • Gene
  • Long gene name
    Thy-1 cell surface antigen
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2001-06-22
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Immunoglobulin like domain containing
    • Ig-like cell adhesion molecule family
    • CD molecules
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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