Anti-Mouse CD34, FITC (Clone MEC14.7) (rat IgG2a)
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Catalog number
MBS520461
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Price
Please ask
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Size
0.3 mg
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Products_type
Antibody
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Products_short_name
[CD34]
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Products_name_syn
[CD34, FITC (Clone MEC14.7) (rat IgG2a); Anti-Mouse CD34 Monoclonal Antibody]
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Other_names
[CD34; Hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell antigen CD34; CD34 antigen; CD34 molecule]
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Products_gene_name
[CD34]
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Other_gene_names
[CD34; CD34]
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Clonality
Monoclonal
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Clone
[MEC 14.7]
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Reactivity
Mouse
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Specificity
CD34 (mucosialin; sialomucin) (Mouse CD34)
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Form
FITC
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Concentration
0.1 mg/ml
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Storage_stability
For all formats, store at 4 degree C. DO NOT FREEZE PE conjugates. For long term storage (Ascites, Purified, Biotin, FITC), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
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Tested_application
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbol
CD34
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Short name
Anti-Mouse CD34, FITC (Clone MEC14 7) ( IgG2a)
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Technique
anti-, Mouse, anti, FITC, antibody to, antibodies, Fluorescein, mouses
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Host
Rat
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Isotype
Rat IgG2a, IgG2a
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Label
FITC
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Species
Rat, Mouses, Rats
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Alternative name
Antibody toMouse CD34 molecule, fluorecein (clonality MEC14.7) (rat IgG2a)
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Alternative technique
murine, antibodies, fluorescine
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Alternative to gene target
CD34 molecule, CD34 and IDBG-106399 and ENSG00000174059 and 947, sulfate binding, Extracellular, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051
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Clone name
MEC14
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Gene info
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Identity
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Gene
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Long gene name
CD34 molecule
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Synonyms gene name
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GenBank acession
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Locus
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Discovery year
1990-09-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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