Anti-Human Complement Factor B/Bb, FITC (clone: M13/12), (Mouse IgG1 K)
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Catalog numberMBS520699
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Price:
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Size0.1 mg
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Products_typeAntibody
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Products_short_name[Complement Factor B/Bb]
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Products_name_syn[Anti-Human Complement Factor B/Bb Monoclonal Antibody]
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Other_names[complement factor B preproprotein; Complement factor B; complement factor B; complement factor B; C3/C5 convertase; Glycine-rich beta glycoprotein; GBG]
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Other_gene_names[CFB; CFB; BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14; BF; BFD; GBG]
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ClonalityMonoclonal
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Clone[M13/12]
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ReactivityHuman
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SpecificityComplement Factor B/Bb
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FormBiotin/FITC/PE conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
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Concentration0.1 mg/ml
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Storage_stabilityFor all formats, store at 4 degree C. DO NOT FREEZE PE conjugates. For long term storage (Purified, Biotin, FITC), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
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Tested_applicationWestern Blot (WB)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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TestMyBioSource supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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GeneM13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
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Latin nameMus musculus
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Gene target
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Short nameAnti- Complement Factor B/Bb, FITC (clone: M13/12), (Mouse IgG1 K)
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Techniqueanti-Human, anti-, Mouse, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein, mouses
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HostMouse
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IsotypeIgG1 K, IgG1
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LabelFITC
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SpeciesMouse, Humans, Mouses
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Alternative nameAntibody toHuman Complement Factor B/Bb, fluorecein (clonality: M13/12), (Mouse IgG1 K)
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Alternative techniquemurine, antibodies, fluorescine
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MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data