Anti-Human (CD25), FITC (clone: YTH 906.9HL), (Rat IgG2b)
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Catalog number
MBS520655
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Price
455.86 USD
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Size
0.1 mg
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Products_type
Antibody
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Products_short_name
[CD25]
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Products_name_syn
[CD25, FITC (clone: YTH 906.9HL), (Rat IgG2b); Anti-Human CD25 (IL-2Ralpha) Monoclonal Antibody]
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Other_names
[CD25; Peripheral myelin protein 22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein]
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Products_gene_name
[CD25]
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Other_gene_names
[Pmp22; Cd25; Pmp-22; PMP-22; SAG]
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Clonality
Monoclonal
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Clone
[YTH 906.9HL]
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Reactivity
Human
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Specificity
CD25 (This antibody is specific for human CD25.)
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Form
FITC
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Concentration
0.1 mg/ml
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Storage_stability
For all formats, store at 4 degree C. DO NOT FREEZE APC and PE conjugates. For long term storage (Purified, Biotin and FITC), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
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Tested_application
Flow Cytometry (FC/FACS), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
MyBioSource supplies antibodies that are for research of human proteins.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
ISG20, IL2RA
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Short name
Anti- (CD25), FITC (clone: YTH 906 9HL), ( IgG2b)
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Technique
anti-Human, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, Fluorescein
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Host
Rat
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Isotype
Rat IgG2b, IgG2b
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Label
FITC
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Species
Rat, Humans, Rats
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Alternative name
Antibody toHuman (CD25), fluorecein (clonality: YTH 906.9HL), (Rat IgG2b)
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Alternative technique
antibodies, fluorescine
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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