Anti-DYKDDDDK-Tag mAb (Agarose conjugated) (Same as Sigma's Anti-FLAG)

• Catalog number
  MBS435026
• Price
  Please ask
• Size
  0,5 mL

• Other size
  please contact us to order other different size
• Properties
  The purest agarose was used in the production of Anti-DYKDDDDK-Tag mAb (Agarose conjugated) (Same as Sigma's Anti- ) by MyBioSource. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Agarose, Flag
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.

• Gene target
  DYKDDDDK-Tag   mAb   conjugated   Same   Sigma's  
• Short name
  Anti-DYKDDDDK-Tag mAb (Agarose conjugated) (Same as Sigma's Anti- )
• Technique
  Agarose, anti-, anti, agaroses, antibody to, antibodies, antibody Conjugates, Tags
• Label
  tag
• Alternative name
  Antibody toDYKDDDDK-detection labelled monoclonal (molecular sieve coupled) (Same as Sigma's Antibody toFLAG)
• Alternative technique
  separation, antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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