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Long name
Recombinant Human Mediator of RNA polymerase II transcription subunit 10 (MED10)
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Alternative names
mediator of RNA polymerase II transcription subunit 10; Mediator of RNA polymerase II transcription subunit 10; mediator of RNA polymerase II transcription subunit 10; TRG-17; TRG-20; transformation-related gene 17 protein; transformation-related gene 20 protein; mediator of RNA polymerase II transcription, subunit 10; mediator complex subunit 10; Mediator complex subunit 10; Transformation-related gene 17 protein; TRG-17; Transformation-related gene 20 protein; TRG-20
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Gene name
MED10
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Other gene names
MED10; MED10; L6; NUT2; TRG20; TRG-17; TRG-20
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General description
Mediator of RNA polymerase II transcription subunit 10 (MED10) is a recombinant protein expressed in Yeast . The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.
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Product category
Recombinant Proteins
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Expression system
Yeast
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Available also expressed in
E Coli ; Yeast ; Baculovirus ; Mammalian Cell
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Purity
Greater than 90% (determined by SDS-PAGE)
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Form
Lyophilized protein
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Storage
This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.
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Applications
This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Description
The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.