Shigella sp. (FITC) Antibody
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Catalog number
MBS611547
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Price
Please ask
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Size
1 mililiter
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Alternative name1
Anti-Shigella sp. (FITC)
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Alternative name2
Shigella sp. (FITC)
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Alternative name3
Shigella sp. (FITC)
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Alternative name4
Shigella sp.
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Alternative name5
Anti -Shigella sp. (FITC)
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Other names
N/A
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
IgG
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Clone
Polyclonal antibody
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Host organism
Rabbit
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Species reactivity
N/A
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Specificity and cross reactivity
Polyvalent for Shigella antigens. Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae.
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Purification method
Affinity Purified by Protein A affinity chromatography.
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Form Appearance
Supplied as a liquid in PBS, pH 7.2, BSA (10mg/ml), 0.1% sodium azide. Conjugated to FITC.
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Concentration
N/A
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Storage and shipping
Store at 4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and For optimal long term storage, productone should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months productone should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
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Tested for
Immunofluorescence (IF)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
Shigella sp. (FITC) Antibody
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Shigella sp. (fluorecein) (antibody to-)
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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