-
Alternative name1
Anti-Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog Homeobox)
-
Alternative name2
Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog Homeobox)
-
Alternative name3
Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog Homeobox)
-
Alternative name4
Nanog
-
Alternative name5
Anti -Nanog (Early Embryo Specific Expression NK Family Gene, Embryonic Stem Cell Specific Homeobox Protein, ENK, FLJ12581, FLJ40451, Homeobox Transcription Factor Nanog, Nanog Homeobox)
-
Other names
NANOG; Homeobox protein NANOG; homeobox protein NANOG; hNanog; OTTHUMP00000231725; OTTHUMP00000231727; OTTHUMP00000238800; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Homeobox transcription factor Nanog
-
Gene name
N/A
-
Gene name synonims
N/A
-
Other gene names
NANOG; NANOG; NANOG; N/A
-
Category
Antibodies
-
Clonality
Polyclonal
-
Immunoglobulin isotype
N/A
-
Clone
Polyclonal antibody
-
Host organism
Host: Goat; Source: Human
-
Species reactivity
Canine, Human, Mouse, Rat
-
Specificity and cross reactivity
This antibody is specific to the NANOG protein. Species Crossreactivity: Human, mouse, rat, and canine. Other species not yet tested.
-
Purification method
Affinity Purified by immunoaffinity chromatography.
-
Form Appearance
Tris saline, pH7.3 with 0.5% bovine serum albumin.
-
Concentration
N/A
-
Storage and shipping
Store at 4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot For optimal long term storage, productone should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months productone should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
-
Tested for
ELISA (EL/EIA), Western Blot (WB)
-
Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
-
Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
Additional description
For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
-
Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
