-
Alternative name1
Anti-HES4 (Hairy Family Helix-loop-helix Transcription Factor) (Biotin)
-
Alternative name2
HES4 (Hairy Family Helix-loop-helix Transcription Factor) (Biotin)
-
Alternative name3
HES4 (Hairy Family Helix-loop-helix Transcription Factor) (Biotin)
-
Alternative name4
HES4
-
Alternative name5
Anti -HES4 (Hairy Family Helix-loop-helix Transcription Factor) (Biotin)
-
Other names
hairy and enhancer of split 4 (Drosophila); Transcription factor HES-4; transcription factor HES-4; hHES4; bHLH factor Hes4; OTTHUMP00000221118; class B basic helix-loop-helix protein 42; hairy and enhancer of split 4 (Drosophila); Class B basic helix-loop-helix protein 42; bHLHb42; Hairy and enhancer of split 4; bHLH factor Hes4
-
Gene name
N/A
-
Gene name synonims
N/A
-
Other gene names
HES4; HES4; bHLHb42; HES4; BHLHB42
-
Category
Antibodies
-
Clonality
Polyclonal
-
Immunoglobulin isotype
IgG
-
Clone
Polyclonal antibody
-
Host organism
Host: Sheep; Source: Human
-
Species reactivity
Human
-
Specificity and cross reactivity
Selected for use as a detection antibody in human HES-4 Western Blots.
-
Purification method
Affinity Purified by human HES-4 affinity chromatography and then biotinylated.
-
Form Appearance
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is us
-
Concentration
N/A
-
Storage and shipping
Lyophilized powder Store at 4 degrees Celsius for short term storage.. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and For optimal long term storage, productone should be kept at -20 degrees Celsius. Reconstituted product is stable for 12 months productone should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
-
Tested for
Western Blot (WB)
-
Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
-
Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
Conjugation
Biotinylated
-
Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
