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Alternative name1
Anti-Goat Anti-Mouse IgG2a, Human ads-APC/Cy7
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Alternative name2
Caprine Anti-Murine IgG2a, Human ads-APC/Cy7
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Alternative name3
Caprinae Anti-Mus musculus IgG2a, Homo sapiens ads-APC/Cy7
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Alternative name4
Goat Anti-Mouse IgG2a (gamma 2a chain specific)
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Alternative name5
Goat Anti-Mouse IgG2a, Human ads - Allophycocyanin/Cyanine7-Labeled; Goat Anti-Mouse IgG2a (gamma 2a chain specific)
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Other names
N/A
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Category
Secondary Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
IgG
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Clone
Polyclonal antibody
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Host organism
Goat
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Species reactivity
Mouse
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Specificity and cross reactivity
Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
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Purification method
Affinity chromatography on mouse IgG2a covalently linked to agarose.
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Form Appearance
Allophycocyanin - Cyanine 7 (APC - CY7) Conjugate
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Concentration
N/A
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Storage and shipping
The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at The items should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC), rhodamine (TRITC), Texas Red (TXRD), and Cyanine 5 (CY 5) conjugates are supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at The items should be kept in the range of 1-7 degrees Celsius. or long-term productone should be stored at -20 degrees Celsius.. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at The items should be kept in the range of 1-7 degrees Celsius. or long-term productone should be stored at -20 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at The items should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at The items should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3 and a stabilizing agent. Store at The items should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and Allophycocyanin-Cyanine 7 (APC-CY 7) conjugates are supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at The items should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
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Tested for
ELISA; Immunoblot; Immunostaining
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Conjugated
These antibodies are excite for emission at 650 nm and detected at a 676 nm wavelengths.
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Conjugation
Cy7
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Latin name
Capra aegagrus hircus, Mus musculus
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Gene symbol
APC
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Short name
Goat Anti-Mouse IgG2a, ads-APC/
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Technique
anti-, Mouse, anti, Goat, antibody to, antibodies, goats, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG2a
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Label
APC
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Species
Human, Goats, Humans, Mouses
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Alternative name
caprine Antibody toMouse IgG2a, H. sapiens ads-adenomatous polyposis coli/cyanine 7
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Alternative technique
murine, antibodies, caprine
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Alternative to gene target
adenomatous polyposis coli, BTPS2 and DP2 and DP2.5 and DP3 and GS and PPP1R46, APC and IDBG-37007 and ENSG00000134982 and 324, microtubule plus-end binding, nuclei, Apc and IDBG-134573 and ENSMUSG00000005871 and 11789, BT.25770 and IDBG-644937 and ENSBTAG00000018852 and 533233
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