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Alternative name1
Anti-Rabbit anti-human Replication protein A 32 kDa subunit polyclonal Antibody, Biotin conjugated
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Alternative name2
Rabbit anti-human Replication protein A 32 kDa subunit PAb, Biotin conjugated
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Alternative name3
Lapin anti-Homo sapiens Replication protein A 32 kDa subunit polyclonal Antibody, Biotin conjugated
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Alternative name4
Replication protein A 32 kDa subunit
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Alternative name5
N/A
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Other names
replication protein A 32 kDa subunit isoform 2; Replication protein A 32 kDa subunit; replication protein A 32 kDa subunit; RF-A protein 2; replication factor A protein 2; replication protein A 34 kDa subunit; replication protein A2, 32kDa; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
RPA2; RPA2; REPA2; RPA32; RP-A p32; RP-A p34; REPA2; RPA32; RPA34; RP-A p32; RF-A protein 2; RP-A p34
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
IgG
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Clone
Polyclonal antibody
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Host organism
Rabbit
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Species reactivity
Human
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Specificity and cross reactivity
N/A
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Purification method
Caprylic Acid Ammonium Sulfate Precipitation
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Form Appearance
Liquid
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Concentration
N/A
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Storage and shipping
Shipped at Store productone at +4 degrees Celsius.. Upon delivery aliquot and store productone should be stored at -20 degrees Celsius. or -80 degree C. Avoid repeated freeze.
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Tested for
Western Blot (WB), ELISA (EIA)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The kilo Daltons subunit weight of Anti- protein A 32 subunit PAb, conjugated compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
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Test
MBS Polyclonals supplies antibodies that are for research of human proteins. Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated