V5 epitope tag (Pk-tag, paramyxovirus, SV5) (Biotin) Antibody

• Catalog number
  GEN605294
• Price
  Please ask
• Size
  100ug

• Also known as
  V5 epitope tag
• Other names
  N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG2a
• Clone
  3H3115
• Host organism
  Mouse (Mus musculus)
• Species reactivity
  N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined. ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  Affinity Purified by Protein G affinity chromatography.
• Form Appearance
  Purified IgG conjugated to Biotin. Supplied as a liquid with 0.1% sodium azide.
• Concentration
  N/A
• Storage and shipping
  Store the antibody at -20 degrees Celsius.
• Tested applications
  ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Properties
  If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  epitope   tag   Pk-tag   paramyxovirus   SV5  
• Short name
  V5 epitope tag (Pk-tag, paramyxovirus, SV5) ( ) Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for, Tags
• Label
  tag
• Alternative name
  Anti-V5 epitope tag (Pk-tag, paramyxovirus, SV5) (Biotin)
• Alternative technique
  antibodies

MeSH Data

• Name
  Epitope Mapping
• Concept
  Scope note: Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry.
• Tree numbers
  • E05.478.274
  • E05.601.690.300
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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