Thomsen Friedenreich Antigen

• Catalog number
  GEN600703
• Price
  Please ask
• Size
  1 mililiter

• Also known as
  Thomsen Friedenreich Antigen
• Other names
  N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgM
• Clone
  5F150
• Host organism
  Mouse (Mus musculus)
• Source organism
  Human (Homo sapiens)
• Species reactivity
  Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  Recognizes the human Thomsen Friedenreich Antigen at ~155kD. Binds to asialoglycophorin but not to glycophorin. Agglutinates neuraminidase-treated but not untreated human erythrocytes. Positive reactions with primary breast cancer, colorectal cancer and metastases derived therefrom. Normal colonic adjacent tissue is negative. Breast epithelium (luminal membranes) may react. Positive staining with acute myelogeneous leukemia cells (HL-60, KG-1). Specific binding with both anomers of TF as well as with the TFb-related asialo-GM1. ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  Supernatant
• Form Appearance
  Supplied as a lyophilized powder, 0.01% sodium azide. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, 0.01% sodium azide.
• Concentration
  N/A
• Storage and shipping
  Lyophilized powder may be stored the antibody should be stored at -20 degrees Celsius.. Stable for 12 months the antibody should be stored at -20 degrees Celsius.. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Reconstituted product is stable for 12 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
• Tested applications
  Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Description
  Antigens are peptides or recombinant or native dependent on the production method.

• Gene target
  Thomsen   Friedenreich  
• Short name
  Thomsen Friedenreich Antigen
• Technique
  antigen, antigenes
• Alternative name
  Anti-Thomsen Friedenreich Antigen
• Alternative technique
  antigenes

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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