Rat anti Mouse kappa light chain antibody (biotin)

  • Also known as
    Rat anti Mouse kappa light chain (biotin)
  • Other names
    N/A
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    LOC100037688; N/A; N/A; N/A
  • Gene name synonims
    LOC100037688; N/A; N/A; N/A
  • Other gene names
    LOC100037688; N/A; N/A; N/A
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG2a kappa
  • Clone
    LO-MK-1
  • Host organism
    Rat (Rattus norvegicus)
  • Species reactivity
    N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    Murine; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    PBS with 0.1% NaN3 and 50% glycerol.
  • Concentration
    1.0 mg/ml
  • Storage and shipping
    Keep the antibody after aliquoting at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies, thus reducing their activity. It is recommended to minimize such cycles as much as possible.
  • Tested applications
    ELISA (EIA), Immunohistochemistry (IHC)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • French translation
    anticorps
  • Gene target
  • Short name
    anti Mouse kappa light chain antibody ( )
  • Technique
    anti mouse, Antibody, light, Mouse, anti, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, mouses
  • Host
    Rat
  • Label
    Biotin
  • Species
    Mouse, Mouses, Rats
  • Alternative name
    Anti-Rat anti Mouse kappa light chain antibody (biotin)
  • Alternative technique
    antibodies, murine
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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