RAT ANTI MOUSE CD70:FITC Antibody
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Catalog numberGEN210941
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Price:
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Size100ug
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Also known asCD70
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Other namesCD70 antigen; CD70 antigen; CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7; CD70 antigen; CD27 ligand; CD27-L; Tumor necrosis factor ligand superfamily member 7
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameCD70
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Gene name synonimsN/A
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Other gene namesCd70; Cd70; Cd27l; CD27LG; Tnfsf7; Cd27l; Cd27lg; Tnfsf7; CD27-L
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ClonalityMonoclonal
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Immunoglobulin isotypeIgG2b
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CloneFR70
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Host organismRat (Rattus norvegicus)
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Species reactivityMouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityCD70 This item recognises mouse CD70, a 30-33kD type II transmembrane protein belonging to the TNF superfamily. CD70 is not expressed on resting T and B lymphocytes but expression is induced on activation. Interaction of CD70 with its ligand CD27 is reported to play an important role in T-cell co-stimulation and the regulation of T-dependant B-cell activation and antibody production. Clone FR70 is reported to block the functions of CD70 and has been used to block the interactions between CD27 and CD70 ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form AppearanceFITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
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ConcentrationIgG concentration 0.1 miligram per 1 mililiter
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Storage and shippingStore the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applicationsFlow Cytometry (FACS)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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PropertiesIf you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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French translationanticorps
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Gene target
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Gene symbolCD70
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Short nameANTI MOUSE CD70:FITC Antibody
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Techniqueanti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
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HostRat
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LabelFITC
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SpeciesMouse, Mouses, Rats
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Alternative nameAnti-RAT ANTI MOUSE CD70:FITC
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetCD70 molecule, CD27L and CD27LG and TNFSF7, CD70 and IDBG-21699 and ENSG00000125726 and 970, protein binding, Extracellular, Cd70 and IDBG-193725 and ENSMUSG00000019489 and 21948, CD70 and IDBG-643945 and ENSBTAG00000009752 and 522074
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Gene info
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Identity
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Gene
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Long gene nameCD70 molecule
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Synonyms gene
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Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 7
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-11-08
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Entrez gene record
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Pubmed identfication
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Classification
- CD molecules
- Tumor necrosis factor superfamily
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data