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Also known as
Myosin, Fast Skeletal
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Other names
myosin light chain 1, skeletal muscle isoform; Myosin light chain 3, skeletal muscle isoform; myosin light chain 1, skeletal muscle isoform; MLC-1; MLC-3; myosin a1; light polypeptide 1; myosin light chain 1f; myosin light chain 3f; myosin light chain A1; myosin light chain A2; alkali myosin light chain 1; alkali myosin light chain 3; myosin light chain alkali 1; myosin light chain alkali 2; skeletal-muscle myosin L-1 light chain; skeletal-muscle myosin L-4 light chain; myosin light chain 3, skeletal muscle isoform; myosin, light polypeptide 1, alkali; skeletal, fast; myosin, light chain 1, alkali; skeletal, fast; Alkali myosin light chain 3; MLC-3; Myosin light chain 3f; Myosin light chain alkali 2; Myosin light chain A2; Skeletal-muscle myosin L-4 light chain
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
MYL1; MLC-3; fast; alkali; myosin; skeletal; MLC-3
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Gene name synonims
MYL1; MLC-3; fast; alkali; myosin; skeletal; MLC-3
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Other gene names
MYL1; MLC-3; fast; alkali; myosin; skeletal; MLC-3
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1,k
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Clone
8L878
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Host organism
Mouse (Mus musculus)
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Source organism
Rabbit (Oryctolagus cuniculus)
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Species reactivity
Bovine, Chicken, Feline, Guinea Pig, Human (Homo sapiens), Mouse (Mus musculus), Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes an epitope in adult rat skeletal myosin heavy chains IIa, IIb and IId (IIx) (in fast-twitch fibers)(1). It also reacts with neonatal but not with embryonal cardiac myosin heavy chains(1). It does not cross react with non-muscle, and smooth muscle myosins. A similar reactivity pattern is observed in human, mouse and chicken skeletal muscle preparations.(2-7). This antibody localizes an epitope on the myosin chain that is stable to the routine formalin fixation. Anti-skeletal myosin to fast twitch skeletal myosin may be used for detecting cross-striated muscle differentiation in tumors.Monoclonal Anti- Myosin (Skeletal, Fast) is useful not only in fiber typing but also in detection of myogenic tumors (10). It enables retrospective as well as prospective fiber typing using formalin-fixed, paraffin-embedded material Species Crossreactivity: Crossreacts with Human, Rabbit, Rat, Mouse, bovine, Cat, Guinea pig and Chicken.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Purified
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Form Appearance
20 mM tris-borate, 150 mM sodium chloride
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested applications
Western Blot (WB), Immunohistochemistry (IHC)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
Myosin, Fast Skeletal (A1 Catalytic, A2 Catalytic, Alkali Myosin Light Chain 1, Alkali Myosin Light Chain 3, DKFZp779C0757, Fast Skeletal Myosin Alkali Light Chain 1, Fast Skeletal Myosin Light Chain 2, MGC13450, MLC1F, MLC3F, Myosin Light Chain 1 Alkali Antibody
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Technique
Antibody, light, antibodies against human proteins, antibodies for
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Alternative name
Anti-Myosin, Fast Skeletal (A1 Catalytic, A2 Catalytic, Alkali Myosin Light Chain 1, Alkali Myosin Light Chain 3, DKFZp779C0757, Fast Skeletal Myosin Alkali Light Chain 1, Fast Skeletal Myosin Light Chain 2, MGC13450, MLC1F, MLC3F, Myosin Light Chain 1 Alkali
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Alternative technique
antibodies
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