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Also known as
MyoD1
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Other names
myoblast determination protein 1; Myoblast determination protein 1; myoblast determination protein 1; myf-3; myogenic factor 3; class C basic helix-loop-helix protein 1; myogenic differentiation 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
MYOD1
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Gene name synonims
N/A
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Other gene names
MYOD1; MYOD1; PUM; MYF3; MYOD; bHLHc1; BHLHC1; MYF3; MYOD; bHLHc1; Myf-3
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1, kappa
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Clone
5.8A
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus), Chicken.; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
N/A
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Concentration
N/A
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Storage and shipping
Store the antibody at +4C. Temperature variations in the range of 1 to 7 degrees Celsius are tolerable. We recommend NOT to freeze the antibody. Antibody is stable for 24 months.
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Tested applications
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH)
