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Also known as
TPSAB1 / Mast Cell Tryptase
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Other names
tryptase alpha/beta-1; Tryptase alpha/beta-1; tryptase alpha/beta-1; tryptase I; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase alpha-1; tryptase alpha II; mast cell beta I tryptase; mast cell alpha II tryptase; tryptase alpha/beta 1; Tryptase I; Tryptase alpha-1
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
TPSAB1
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Gene name synonims
N/A
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Other gene names
TPSAB1; TPSAB1; TPS1; TPS2; TPSB1; TPS1; TPS2; TPSB1; Tryptase-1
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
N/A
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Host organism
Mouse (Mus musculus)
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Species reactivity
Cat, Dog, Human (Homo sapiens), Monkey; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes human mast cell tryptase, both alpha and beta isoforms. The antibody is an excellent marker for mast cells and does not bind to any other cell type in Immunohistochemistry. Species cross-reactivity: feline, canine and monkey.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Protein G Purified
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Form Appearance
PBS, 0.09% sodium azide
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Concentration
1 miligram per 1 mililiter
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Storage and shipping
For long term storage, keep the antibody at -20 degrees Celsius. Short term storage of the antibody can be done at +4 degrees Celsius. Cycles of freezing/defrosting can damage the peptide chains of the antibodies thus reducing the activity.
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Tested applications
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), ELISA (EIA)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Description
For cells, cell lines and tissues in culture till half confluency.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps