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Also known as
CYCS / Cytochrome c
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Other names
cytochrome c; Cytochrome c; cytochrome c; cytochrome c, somatic; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CYCS
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Gene name synonims
N/A
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Other gene names
CYCS; CYCS; CYC; HCS; THC4; CYC
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2b
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Clone
7H8.2C12
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Host organism
Mouse (Mus musculus)
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Species reactivity
Bovine, Guinea pig, Hamster, Horse, Human (Homo sapiens), Monkey, Mouse (Mus musculus), Pig, Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus), Sheep; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Detects an ~14kD protein, corresponding to the apparent molecular mass of Cytochrome c on Western blots with samples from human, mouse, rat, bovine, guinea pig, hamster, monkey, rabbit and sheep origins. This antibody detects denatured Cytochrome c in applications such as Western blot, it does not recognize the native protein. The epitope of this antibody was mapped to a region within amino acids 93-104 of horse Cytochrome.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Protein G Purified
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Form Appearance
PBS, pH 7.2, 50% glycerol, 0.09% azide
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Concentration
1 miligram per 1 mililiter
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Storage and shipping
Store at +4 degrees Celsius for short term storage. For long term storage aliquot and keep the antibody at -20 degrees Celsius. Freeze-thaw cycles should be avoided in order to retain the activity of the antibody.
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Tested applications
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
CYCS
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Short name
Mouse Monoclonal [clone 7H8 2C12] (IgG2b) CYCS / Cytochrome c Antibody
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG2b
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Species
Horse, Horses, Mouses
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Alternative name
Anti-Mouse Monoclonal [clone 7H8.2C12] (IgG2b) to Horse CYCS / Cytochrome c
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Alternative technique
antibodies, murine
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Alternative to gene target
cytochrome c, somatic, CYCS and IDBG-10008 and ENSG00000172115 and 54205, electron transporter, nuclei
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Clone name
7H8
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