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Also known as
PAG
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Other names
phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Csk-binding protein; transmembrane phosphoprotein Cbp; transmembrane adapter protein PAG; transmembrane adaptor protein PAG; phosphoprotein associated with glycosphingolipid microdomains 1; Csk-binding protein; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
PAG
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Gene name synonims
N/A
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Other gene names
PAG1; PAG1; CBP; PAG; CBP; PAG
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2a
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Clone
MEM-255
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
PAG This item specifically recognizes the transmembrane adaptor protein known as Protein Associated with Glycosphingolipid-enriched microdomains (PAG), _x000D_an epitope (aa 235-280) located in the cytoplasmic domain of csk-binding protein (Cbp), which localizes in membrane lipid rafts. _x000D__x000D_The engagement of immunoreceptors with natural ligands or antibodies is the initiating factor in a long line of events down a cytosolic signaling cascade. _x000D_The transmission of signals along the various pathways within this cascade requires the phosphorylation of various substrates, by members of the src and syk families of protein tyrosine kinases (PTKs). _x000D__x000D_Evidence has shown that by binding to the SH2 domain of the PTK csk, a major negative regulator of src-family kinases, PAG acts not only acts as a 'co-factor' for relocating csk to substrate rich microdomains, but also increases the affinity of csk for src-family kinases, through the formation of PAG-csk complexes.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Purified (Purified IgG - liquid)
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Concentration
Ig concentration 1.0 mg/ml
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
MBS Monoclonals supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps