Monoclonal antibody against DYKDDDDK tag, Clone Flag.C2
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Catalog number
GEN592333
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Price
Please ask
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Size
500ug
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Also known as
DYKDDDDK tag
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Other names
N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
Mouse, IgG1 Kappa
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Clone
N/A
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Host organism
Mouse (Mus musculus)
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Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
This monoclonal antibody binds to DYKDDDDK-tagged recombinant protein.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Purified primary antibodies
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Form Appearance
Lyophilized from a solution in 0.01M PBS, pH 7.2 with 0.05% sodium azide
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Concentration
N/A
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Storage and shipping
Aliquot and store the antibody should be stored at -20 degrees Celsius. for long term storage. Avoid repeated freeze and thaw cycles.
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Tested applications
Western Blot (WB)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Short name
Monoclonal antibody against DYKDDDDK tag, Clone .C2
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Technique
Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
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Label
tag
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Alternative name
Anti-Mouse monoclonal antibody against DYKDDDDK tag, Clone Flag.C2
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Alternative technique
antibodies
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Alternative to gene target
complement component 2, ARMD14 and CO2, C2 and IDBG-124824 and ENSG00000166278 and 101928623,717, protein binding, Extracellular, C2 and IDBG-174547 and ENSMUSG00000024371 and 12263, C2 and IDBG-633680 and ENSBTAG00000007450 and 515440
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Clone name
.C2
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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