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Also known as
CD3
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Other names
T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3 antigen, epsilon polypeptide; T-cell surface antigen T3/Leu-4 epsilon chain
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CD3
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Gene name synonims
N/A
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Other gene names
Cd3e; Cd3e; CD3; T3e; AI504783; CD3epsilon; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG
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Clone
145-2C11
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Host organism
Hamster
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
CD3 This item detects CD3 epsilon (CD3e), a 20kDa transmembrane protein also known as CD3 or T3. CD3e is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell. _x000D__x000D_CD3e is primarily expressed on T cells, NK-T cells and at different levels on thymocytes during T cell differentiation. _x000D__x000D_The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Purified (Purified IgG - liquid)
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Concentration
IgG concentration 0.5 mg/ml
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
Immunohistology - Frozen (IHC), Flow Cytometry (FACS), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps