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Also known as
E2F3 Transcription Factor
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Other names
E2F transcription factor 3; Transcription factor E2F3; transcription factor E2F3; OTTHUMP00000018012; E2F transcription factor 3; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
E2F3; E2F3; E2F-3; KIAA0075; MGC104598; DKFZp686C18211; KIAA0075
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Gene name synonims
E2F3; E2F3; E2F-3; KIAA0075; MGC104598; DKFZp686C18211; KIAA0075
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Other gene names
E2F3; E2F3; E2F-3; KIAA0075; MGC104598; DKFZp686C18211; KIAA0075
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Clonality
Monoclonal
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Immunoglobulin isotype
N/A
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Clone
0.T.32
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes human E2F-3 at ~58kD. An additional band may be detected at ~44kD with longer exposures. Species Crossreactivity: mouse, weak crossreactivity with rat.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein A affinity chromatography.
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Form Appearance
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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Tested applications
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
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Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
