Mouse IFN-gamma/IL-10 FluoroSpotPLUS kit

Categories

FluoroSpot Kits
Description

Pre-coated plates enable a more rapid assay with minimized variability.
Enzyme Conjugate

NA
Format

pre-coated plates
Kit Detection Type

NA
Application

FluoroSpot
Reactivities

mouse
Analytes

IFNG, IL10
Contents

BAM-conjugated detection mAb (R4-6A2),Biotinylated detection mAb (51E6),Anti-BAM-490,SA-550,Anti-CD28 mAb ,Pre-coated FluoroSpot plates (mAbs AN18 and MT60),Fluorescence enhancer
Datasheet
Product Header

FluoroSpot kit with pre-coated plates for enumeration of cells secreting IFN-γ and IL-10
GeneID

NA
Shelf Life

12 months
Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name

Mus musculus

Gene target

IFN-gamma/IL-10 FluoroSpotPLUS kit
Gene symbol

IFNA1, IFNA10, IL10, IFNA6, IFNA21, IFNA5, IFNA16, IFNA14, IFNA8, IFNA7
Short name

Mouse IFN-gamma/IL-10 FluoroSpotPLUS kit
Technique

Mouse, mouses
Host

mouse
Species

Mouse, Mouses
Alternative name

Mouse Interferon-g/Interleukin-10 FluoroSpotPLUS reagent
Alternative technique

murine, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Identity

HGNC:5962
Gene

IL10
Long gene name

interleukin 10
Synonyms
- CSIF
- TGIF
- IL10A
- IL-10
Synonyms name
- cytokine synthesis inhibitory factor
- T-cell growth inhibitory factor
GenBank acession
- M57627
Locus

1q32.1
Discovery year

1991-10-31
Entrez gene record

3586
Pubmed identfication
- 9162098
RefSeq identity
- NM_000572
Classification
- Interleukins
VEGA ID

OTTHUMG00000036386
Locus Specific Databases
- LRG_1230

Name

Enzyme-Linked Immunospot Assay
Concept

Scope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data