anti-mouse IL-10 mAb MT60, purified

Categories

Antibodies
Description

This monoclonal antibody enables sensitive and specific detection of mouse IL-10 in immunoassays such ELISA and ELISpot.
Recommandation

We recommend MT60 as capture antibody in ELISA and ELISpot in combination with biotinylated detection antibody 51E6.
Antibody Clone

MT60
Enzyme Conjugate

unconjugated
Format

purified
Application

ELISA, ELISpot
Reactivities

mouse
Analytes

IL10
Specificity

Native and recombinant IL-10
Contents

MT60, purified, supplied at 0.5 mg/ml in PBS with 0.02% sodium azide
Datasheet
Product Header

Monoclonal antibody specific for IL-10
Shelf Life

18 months
Additional description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name

Mus musculus

Gene target

IL-10 mAb MT60
Gene symbol

IL10, PIRC49, SNORD115-10, SNORD116-10, IGHV3OR16-10, IGKV2OR2-10, IL36G
Short name

anti-mouse IL-10 mAb MT60, purified
Technique

purified, anti-, Mouse, anti, antibody to, antibodies, mouses, purifications
Host

Rat
Isotype

IgG2a
Species

Mouse, Mouses
Alternative name

Antibody tomouse Interleukin-10 monoclonal MT60, enriched
Alternative technique

pure, murine, antibodies

Identity

HGNC:5962
Gene

IL10
Long gene name

interleukin 10
Synonyms
- CSIF
- TGIF
- IL10A
- IL-10
Synonyms name
- cytokine synthesis inhibitory factor
- T-cell growth inhibitory factor
GenBank acession
- M57627
Locus

1q32.1
Discovery year

1991-10-31
Entrez gene record

3586
Pubmed identfication
- 9162098
RefSeq identity
- NM_000572
Classification
- Interleukins
VEGA ID

OTTHUMG00000036386
Locus Specific Databases
- LRG_1230

Identity

HGNC:5806
Gene

IGKV2OR2-10
Long gene name

immunoglobulin kappa variable 2/OR2-10 (pseudogene)
Synonyms gene name
- immunoglobulin kappa variable 2/OR2-10
- immunoglobulin kappa variable 2/OR2-10 pseudogene
Synonyms
- IGKV2/OR2-10
GenBank acession
- X51886
Locus

2q11.2
Discovery year

2000-04-18
Entrez gene record

28855
RefSeq identity
- NG_011661
Classification
- Immunoglobulin kappa (IGK) orphons

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data