anti-human IL-10 mAb 12G8, biotinylated

Categories

Antibodies
Description

This monoclonal antibody enables sensitive and specific detection of human IL-10 in immunoassays such as ELISA and ELISpot.
Recommandation

We recommend 12G8 as detection antibody in ELISA and ELISpot in combination with capture antibody 9D7.
Antibody Clone

12G8
Enzyme Conjugate

biotin
Format

biotinylated
Application

ELISpot, ELISA
Reactivities

human
Analytes

IL10
Specificity

Native and recombinant human IL-10
Contents

12G8, biotinylated, supplied at 1 mg/ml in PBS with 0.02% sodium azide
Datasheet
Product Header

Monoclonal antibody specific for IL-10
Shelf Life

18 months
Additional description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
Test

Mabtech supplies antibodies that are for research of human proteins.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Gene target

IL-10 mAb 12G8 biotinylated
Gene symbol

IL10, PIRC49, SNORD115-10, SNORD116-10, IGHV3OR16-10, IGKV2OR2-10, IL36G, IL25
Short name

anti- IL-10 mAb 12G8, biotinylated
Technique

anti-Human, anti-, anti, antibody to, antibodies, antibodies against human proteins
Host

Rat
Isotype

IgG2a
Species

Human, Humans
Alternative name

Antibody tohuman Interleukin-10 monoclonal 12G8, biotinylated
Alternative technique

antibodies

Identity

HGNC:5962
Gene

IL10
Long gene name

interleukin 10
Synonyms
- CSIF
- TGIF
- IL10A
- IL-10
Synonyms name
- cytokine synthesis inhibitory factor
- T-cell growth inhibitory factor
GenBank acession
- M57627
Locus

1q32.1
Discovery year

1991-10-31
Entrez gene record

3586
Pubmed identfication
- 9162098
RefSeq identity
- NM_000572
Classification
- Interleukins
VEGA ID

OTTHUMG00000036386
Locus Specific Databases
- LRG_1230

Identity

HGNC:5806
Gene

IGKV2OR2-10
Long gene name

immunoglobulin kappa variable 2/OR2-10 (pseudogene)
Synonyms gene name
- immunoglobulin kappa variable 2/OR2-10
- immunoglobulin kappa variable 2/OR2-10 pseudogene
Synonyms
- IGKV2/OR2-10
GenBank acession
- X51886
Locus

2q11.2
Discovery year

2000-04-18
Entrez gene record

28855
RefSeq identity
- NG_011661
Classification
- Immunoglobulin kappa (IGK) orphons

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data