dsGreen for Real-Time PCR, 100×, 10 mL

Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
Description

Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.

Gene target

dsGreen for 100×
Short name

dsGreen for Real-Time PCR, 100×, 10
Technique

Real-time, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
Alternative name

dsGreen to measure Real-Time PCR test kit, 100×, 10 milliliter
Alternative technique

rtpcr, dna-amplification

Name

Real-Time Polymerase Chain Reaction
Concept

Scope note: Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
Tree numbers
- E05.393.620.500.706
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data