dsGreen for Real-Time PCR, 100×, 10 mL
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Catalog number81010
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Price:
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Size10 mL
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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DescriptionReal time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.
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Gene target
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Short namedsGreen for Real-Time PCR, 100×, 10
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TechniqueReal-time, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Alternative namedsGreen to measure Real-Time PCR test kit, 100×, 10 milliliter
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Alternative techniquertpcr, dna-amplification
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MeSH Data
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Name
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ConceptScope note: Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
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Tree numbers
- E05.393.620.500.706
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data