pLVX-NICD-FLAG-Puro(Notch2)
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Vectro Map
The pLVX promotor is combined with a NICD vector sequence and a FLAG gene in the plasmid vector map.
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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Gene target
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Short name
pLVX-NICD- -Puro(Notch2)
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Label
Flag
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Alternative name
pLVX-NICD-FLAG-Puro(notch 2)
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Alternative to gene target
notch 2, AGS2 and HJCYS and hN2, NOTCH2 and IDBG-101546 and ENSG00000134250 and 4853, ligand-activated RNA polymerase II transcription factor binding transcription factor activity, nuclei, Notch2 and IDBG-177579 and ENSMUSG00000027878 and 18129, NOTCH2 and IDBG-634128 and ENSBTAG00000007909 and 513730
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