pIRES2-EGFP-DNM1L-Flag Plasmid
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Catalog number
PVTB01156-2a
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Price
381.63 USD
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Size
2 ug
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Conjugate
The Green Fluorescent Protein (eGFP) of pIRES2- -DNM1L- Plasmid can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbol
DNM1L
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Short name
pIRES2- -DNM1L- Plasmid
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Technique
plasmid, plasmids in 1
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Label
Flag
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Alternative name
pIRES2-EGFP-dynamin 1-like-Flag Plasmid
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Alternative technique
plasmids
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Alternative to gene target
dynamin 1-like, DLP1 and DRP1 and DVLP and DYMPLE and EMPF and HDYNIV, DNM1L and IDBG-26348 and ENSG00000087470 and 10059, protein homodimerization activity, Cell surfaces, Dnm1l and IDBG-136828 and ENSMUSG00000022789 and 74006, DNM1L and IDBG-638362 and ENSBTAG00000011395 and 540892
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Gene info
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Identity
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Gene
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Long gene name
dynamin 1 like
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-04-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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