pGL3-Basic-UHRF1 Enhancer (MT)Reverse Plasmid
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Catalog number
PVTB80048-2e
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Price
381.42 USD
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Size
2 ug
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Description
Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer. Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Goup
reverse transcription
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Gene target
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Gene symbol
UHRF1
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Short name
pGL3- -UHRF1 Enhancer (MT)Reverse Plasmid
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Technique
plasmid, reverse, plasmids in 1
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Alternative name
pGL3-Basic-UHRF1 Enhancer (MT)Reverse Plasmid
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Alternative technique
transctription, plasmids
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Gene info
MeSH Data
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Name
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Concept
Scope note:
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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