pCR-XL-TOPO-IGF1R Plasmid

Quantity:

Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
Kit

Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

Gene target

XL-TOPO-IGF1R
Gene symbol

IRAIN, IGF1R, BCL2L1, BCL11A
Short name

pCR-XL-TOPO-IGF1R Plasmid
Technique

plasmid, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. plasmids in 1
Alternative name

PCR test kit-XL-TOPO-insulin-like growth factor 1 receptor Plasmid
Alternative technique

plasmids, dna-amplification
Alternative to gene target

insulin-like growth factor 1 receptor, CD221 and IGFIR and IGFR and JTK13, IGF1R and IDBG-31432 and ENSG00000140443 and 3480, transferase activity, Cell surfaces, Igf1r and IDBG-192339 and ENSMUSG00000005533 and 16001, IGF1R and IDBG-628659 and ENSBTAG00000021527 and 281848

Quantity:

Identity

HGNC:5465
Gene

IGF1R
Long gene name

insulin like growth factor 1 receptor
Synonyms gene name
- insulin-like growth factor 1 receptor
Synonyms
- JTK13
- CD221
- IGFIR
- MGC18216
- IGFR
GenBank acession
- M69229
Locus

15q26.3
Discovery year

1988-07-07
Entrez gene record

3480
Pubmed identfication
- 1316909
RefSeq identity
- NM_000875
Classification
- Receptor tyrosine kinases
- Fibronectin type III domain containing
- CD molecules
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000149851
Locus Specific Databases
- Global Variome shared LOVD
- LRG_1055

Identity

HGNC:13221
Gene

BCL11A
Long gene name

BAF chromatin remodeling complex subunit BCL11A
Synonyms gene
- EVI9
Synonyms gene name
- ecotropic viral integration site 9
- B-cell CLL/lymphoma 11A (zinc finger protein)
- B cell CLL/lymphoma 11A
- BCL11A, BAF complex component
Synonyms
- BCL11A-XL
- BCL11A-L
- BCL11A-S
- CTIP1
- HBFQTL5
- ZNF856
- SMARCM1
GenBank acession
- AJ404611
Locus

2p16.1
Discovery year

2001-02-28
Entrez gene record

53335
Pubmed identfication
RefSeq identity
- NM_022893
Classification
- Zinc fingers C2H2-type
- BAF complex
VEGA ID

OTTHUMG00000129420
Locus Specific Databases
- The Globin Gene Server

Name

Nucleic Acid Amplification Techniques
Concept

Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
Tree numbers
- E05.393.620
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data