pCR-XL-TOPO-GLI3 Plasmid
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Catalog numberPVTB00149S
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Price:
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Size2 ug
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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KitPlasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbolGLI3, BCL2L1, BCL11A
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Short namepCR-XL-TOPO-GLI3 Plasmid
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Techniqueplasmid, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. plasmids in 1
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Alternative namePCR test kit-XL-TOPO-GLI family zinc finger 3 Plasmid
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Alternative techniqueplasmids, dna-amplification
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Alternative to gene targetGLI family zinc finger 3, ACLS and GCPS and GLI3-190 and GLI3FL and PAP-A and PAPA and PAPA1 and PAPB and PHS and PPDIV, GLI3 and IDBG-13536 and ENSG00000106571 and 2737, metal ion binding, nuclei, Gli3 and IDBG-131686 and ENSMUSG00000021318 and 14634, GLI3 and IDBG-634502 and ENSBTAG00000010671 and 785371
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Gene info
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Identity
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Gene
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Long gene nameGLI family zinc finger 3
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Synonyms gene
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Synonyms gene name
- Greig cephalopolysyndactyly syndrome
- GLI-Kruppel family member GLI3
- glioma-associated oncogene family zinc finger 3
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Synonyms
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Synonyms name
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Locus
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Discovery year1989-05-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameBCL2 like 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- BCL2 family
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameBAF chromatin remodeling complex subunit BCL11A
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Synonyms gene
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Synonyms gene name
- ecotropic viral integration site 9
- B-cell CLL/lymphoma 11A (zinc finger protein)
- B cell CLL/lymphoma 11A
- BCL11A, BAF complex component
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers C2H2-type
- BAF complex
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
- E05.393.620.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data