pCR-BluntII-TOPO-TCF3 Plasmid

Quantity:

Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
Kit

Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

Gene target

BluntII-TOPO-TCF3
Gene symbol

TCF7L1, TCF3
Short name

pCR-BluntII-TOPO-TCF3 Plasmid
Technique

plasmid, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. plasmids in 1
Alternative name

PCR test kit-BluntII-TOPO-transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) Plasmid
Alternative technique

plasmids, dna-amplification
Alternative to gene target

transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47), bHLHb21 and E2A and E47 and ITF1 and TCF-3 and VDIR, TCF3 and IDBG-14881 and ENSG00000071564 and 6929, E-box binding, nuclei, Tcf3 and IDBG-174161 and ENSMUSG00000020167 and 21423, TCF3 and IDBG-645672 and ENSBTAG00000008695 and 530616

Quantity:

Name

Nucleic Acid Amplification Techniques
Concept

Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
Tree numbers
- E05.393.620
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data