pCR-BluntII-TOPO-ROBO1 Plasmid

Quantity:

Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
Kit

Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

Gene target

BluntII-TOPO-ROBO1
Gene symbol

ROBO1
Short name

pCR-BluntII-TOPO-ROBO1 Plasmid
Technique

plasmid, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. plasmids in 1
Alternative name

PCR test kit-BluntII-TOPO-roundabout, axon guidance receptor, homolog 1 (Drosophila) Plasmid
Alternative technique

plasmids, dna-amplification
Alternative to gene target

roundabout, axon guidance receptor, homolog 1 (Drosophila), DUTT1 and SAX3, ROBO1 and IDBG-45469 and ENSG00000169855 and 6091, protein heterodimerization activity, Cell surfaces, Robo1 and IDBG-171072 and ENSMUSG00000022883 and 19876, ROBO1 and IDBG-630648 and ENSBTAG00000009851 and 536815

Quantity:

Identity

HGNC:10249
Gene

ROBO1
Long gene name

roundabout guidance receptor 1
Synonyms gene name
- roundabout (axon guidance receptor, Drosophila) homolog 1
- roundabout, axon guidance receptor, homolog 1 (Drosophila)
Synonyms
- DUTT1
- FLJ21882
- SAX3
GenBank acession
- AF040990
Locus

3p12.3
Discovery year

1998-03-26
Entrez gene record

6091
Pubmed identfication
- 9458045
- 9608531
RefSeq identity
- NM_002941
Classification
- Fibronectin type III domain containing
- Ig-like cell adhesion molecule family
- I-set domain containing
VEGA ID

OTTHUMG00000158843

Name

Nucleic Acid Amplification Techniques
Concept

Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
Tree numbers
- E05.393.620
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data