pCR-BluntII-TOPO-ROBO1 Plasmid
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Catalog numberPVTB00489
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Price:
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Size2 ug
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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KitPlasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Gene target
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Gene symbolROBO1
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Short namepCR-BluntII-TOPO-ROBO1 Plasmid
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Techniqueplasmid, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. plasmids in 1
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Alternative namePCR test kit-BluntII-TOPO-roundabout, axon guidance receptor, homolog 1 (Drosophila) Plasmid
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Alternative techniqueplasmids, dna-amplification
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Alternative to gene targetroundabout, axon guidance receptor, homolog 1 (Drosophila), DUTT1 and SAX3, ROBO1 and IDBG-45469 and ENSG00000169855 and 6091, protein heterodimerization activity, Cell surfaces, Robo1 and IDBG-171072 and ENSMUSG00000022883 and 19876, ROBO1 and IDBG-630648 and ENSBTAG00000009851 and 536815
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Gene info
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Identity
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Gene
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Long gene nameroundabout guidance receptor 1
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Synonyms gene name
- roundabout (axon guidance receptor, Drosophila) homolog 1
- roundabout, axon guidance receptor, homolog 1 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-03-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibronectin type III domain containing
- Ig-like cell adhesion molecule family
- I-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
- E05.393.620
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data