pCR-Blunt II-TOPO-CASP14 Plasmid

Quantity:

Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
Kit

Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

Gene target

Blunt II-TOPO-CASP14
Gene symbol

CASP14, CRABP2, SPINK2, MGST2, IBSP, APOL2, MGAT2, P4HA2, XYLT2, TMED10P1
Short name

pCR-Blunt II-TOPO-CASP14 Plasmid
Technique

plasmid, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. plasmids in 1
Alternative name

PCR test kit-Blunt II-TOPO-caspase 14, apoptosis-related cysteine peptidase Plasmid
Alternative technique

plasmids, dna-amplification
Alternative to gene target

caspase 14, apoptosis-related cysteine peptidase, CASP14 and IDBG-34154 and ENSG00000105141 and 23581, cysteine-type peptidase activity, nuclei, Casp14 and IDBG-169485 and ENSMUSG00000005355 and 12365, CASP14 and IDBG-642581 and ENSBTAG00000021106 and 517658

Quantity:

Identity

HGNC:1502
Gene

CASP14
Long gene name

caspase 14
Synonyms gene name
- caspase 14, apoptosis-related cysteine protease
- caspase 14, apoptosis-related cysteine peptidase
Synonyms
- MICE
- MGC119078
- MGC119079
Synonyms name
- apoptosis-related cysteine protease
Locus

19p13.12
Discovery year

1998-11-09
Entrez gene record

23581
Pubmed identfication
RefSeq identity
- NM_012114
Classification
- Caspases
VEGA ID

OTTHUMG00000183205

Identity

HGNC:11245
Gene

SPINK2
Long gene name

serine peptidase inhibitor Kazal type 2
Synonyms gene name
- serine protease inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)
Synonyms
- HUSI-II
Synonyms name
- acrosin-trypsin inhibitor
GenBank acession
- BC022514
Locus

4q12
Discovery year

1993-12-01
Entrez gene record

6691
Pubmed identfication
- 8428671
RefSeq identity
- NM_021114
Classification
- Serine peptidase inhibitors, Kazal type
VEGA ID

OTTHUMG00000128769

Identity

HGNC:7045
Gene

MGAT2
Long gene name

alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Synonyms gene name
- mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Synonyms
- GNT-II
GenBank acession
- U15128
Locus

14q21.3
Discovery year

1993-02-16
Entrez gene record

4247
Pubmed identfication
- 7635144
RefSeq identity
- NM_002408
Classification
- Mannosyl-glycoprotein N-acetylglucosaminyltransferases
VEGA ID

OTTHUMG00000140271
Locus Specific Databases
- Congenital Disorder of Glycosylation pages

Identity

HGNC:8547
Gene

P4HA2
Long gene name

prolyl 4-hydroxylase subunit alpha 2
Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II
- prolyl 4-hydroxylase, alpha polypeptide II
Synonyms
- C-P4Halpha(II)
Synonyms name
- 4-PH alpha 2
- collagen prolyl 4-hydroxylase alpha(II)
GenBank acession
- U90441
Locus

5q31.1
Discovery year

1999-02-23
Entrez gene record

8974
Pubmed identfication
- 9211872
- 9149945
RefSeq identity
- NM_004199
Classification
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000059647

Identity

HGNC:30754
Gene

TMED10P1
Long gene name

transmembrane p24 trafficking protein 10 pseudogene 1
Synonyms gene
- TMED10P
Synonyms gene name
- transmembrane emp24-like trafficking protein 10 (yeast) pseudogene
- transmembrane emp24-like trafficking protein 10 (yeast) pseudogene 1
Synonyms
- Tmp21-II
GenBank acession
- AJ004914
Locus

8q24.3
Discovery year

2005-08-26
Entrez gene record

286102
Pubmed identfication
- 10376215
RefSeq identity
- XR_002807
VEGA ID

OTTHUMG00000165203

Name

Nucleic Acid Amplification Techniques
Concept

Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
Tree numbers
- E05.393.620
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data